The previous reports demonstrated that ginseng saponins, active ingredient of Panax ginseng, inhibited blood vessel contraction induced by various hormones or high K+. Recently, we demonstrated that 20(R)- and 20(S)-ginsenoside RG©ý regulate ion channel activities with differential manners. The aim of this study was to examine whether ginsenoside RG©ý isomers also show differential effects on swine coronary artery contractionresponses induced by high K+, serotonin (5-HT) or acetylcholine. Treatment of 20(S)- but not 20(R)-ginsenoside RG©ý caused a concentration-dependent relaxation of coronary artery contracted by 25 mM KCl. 20(S)- and 20(R)-ginsenoside RG©ý induced significant relaxations of coronary artery contraction induced by 5-HT (3 ¥ìM) in the presence of endothelium with concentration-dependent manner and, also in the absence of endothelium only 20(S)-ginsenoside RG©ý induced a strong inhibition of coronary artery contraction induced by 5-HT in a concentration-dependent manner. 20(S)-ginsenoside RG©ý caused relaxation of coronary artery in the absence and presence of endothelium. In contrast, treatment of 20(S)- and 20(R)-ginsenoside RG©ý (100 ¥ìM) did not show significant inhibition of coronary artery contraction induced by acetylcholine (0.01 to 30 ¥ìM) in the presence of endothelium, whereas both isomers caused significant inhibition of coronary artery contraction induced by acetylcholine (0.01 to 30 ¥ìM) in the absence of endothelium in a concentration-dependent manner. These findings indicate that 20(S)- or 20(R)-ginsenoside RG©ý exhibits differential relaxation effects of swine coronary artery contractions caused by high K+, acetylcholine, and 5-HT treatment and that this differential vasorelaxing effects of ginsenoside RG©ý isomers also might be dependent on endothelium.
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